Leptin‐like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax)
Identifieur interne : 000447 ( Main/Exploration ); précédent : 000446; suivant : 000448Leptin‐like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax)
Auteurs : Chiara Gambardella [Italie] ; Sara Ferrando [Italie] ; Lorenzo Gallus [Italie] ; Silvia Ravera [Italie] ; Paolo Bianchini [Italie] ; Paola Ramoino [Italie] ; Salvatore Fasulo [Italie] ; Grazia Tagliafierro [Italie]Source :
- Microscopy Research and Technique [ 1059-910X ] ; 2010-08.
English descriptors
- KwdEn :
Abstract
The mammalian hormone, leptin, is mainly synthesized in adipose tissue along with other tissues. Leptin plays a role in numerous processes such as in the control of food intake, homeostasis, immune function and reproduction. In this study, we detected and localized leptin immunoreactivity to the muscle of early juvenile sea bass (Dicentrarchus labrax) by Western blot analysis and immunohistochemistry. A leptin immunopositive band with a molecular weight of ∼16 kDa, corresponding to mammalian leptin, was identified in trunk skeletal muscle homogenate. Furthermore, leptin immunopositive cells were detected in the endomysium of skeletal muscular fibers. These cells showed immunostained cytoplasmic granules and roundish and oval nuclei. The most intense immunostaining was observed in the endomysial space among the superficial red muscular fibers of the trunk. These findings suggest that in early juvenile sea bass, leptin is mostly produced by skeletal muscles. Therefore, during the developmental stage lacking adipose tissue, skeletal muscles can be considered an important source of leptin. As already suggested in mammals, we can hypothesize the potential roles of leptin not only in energy expenditure for muscle contraction but also during muscle differentiation and growth. Microsc. Res. Tech. 73:797–802, 2010. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jemt.20822
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000224
- to stream Istex, to step Curation: 000224
- to stream Istex, to step Checkpoint: 000184
- to stream Main, to step Merge: 000452
- to stream Main, to step Curation: 000447
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Leptin‐like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax)</title><author><name sortKey="Gambardella, Chiara" sort="Gambardella, Chiara" uniqKey="Gambardella C" first="Chiara" last="Gambardella">Chiara Gambardella</name></author><author><name sortKey="Ferrando, Sara" sort="Ferrando, Sara" uniqKey="Ferrando S" first="Sara" last="Ferrando">Sara Ferrando</name></author><author><name sortKey="Gallus, Lorenzo" sort="Gallus, Lorenzo" uniqKey="Gallus L" first="Lorenzo" last="Gallus">Lorenzo Gallus</name></author><author><name sortKey="Ravera, Silvia" sort="Ravera, Silvia" uniqKey="Ravera S" first="Silvia" last="Ravera">Silvia Ravera</name></author><author><name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name></author><author><name sortKey="Ramoino, Paola" sort="Ramoino, Paola" uniqKey="Ramoino P" first="Paola" last="Ramoino">Paola Ramoino</name></author><author><name sortKey="Fasulo, Salvatore" sort="Fasulo, Salvatore" uniqKey="Fasulo S" first="Salvatore" last="Fasulo">Salvatore Fasulo</name></author><author><name sortKey="Tagliafierro, Grazia" sort="Tagliafierro, Grazia" uniqKey="Tagliafierro G" first="Grazia" last="Tagliafierro">Grazia Tagliafierro</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2297E2743CDC00F707CFCA27458F2A01BBEA61BC</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/jemt.20822</idno><idno type="url">https://api.istex.fr/document/2297E2743CDC00F707CFCA27458F2A01BBEA61BC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000224</idno><idno type="wicri:Area/Istex/Curation">000224</idno><idno type="wicri:Area/Istex/Checkpoint">000184</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000184</idno><idno type="wicri:doubleKey">1059-910X:2010:Gambardella C:leptin:like:immunoreactivity</idno><idno type="wicri:Area/Main/Merge">000452</idno><idno type="wicri:Area/Main/Curation">000447</idno><idno type="wicri:Area/Main/Exploration">000447</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Leptin‐like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax)</title><author><name sortKey="Gambardella, Chiara" sort="Gambardella, Chiara" uniqKey="Gambardella C" first="Chiara" last="Gambardella">Chiara Gambardella</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Animal Biology and Marine Ecology, University of Messina, Messina 98166</wicri:regionArea><wicri:noRegion>Messina 98166</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biology, University of Genoa, LIBiOM, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author><author><name sortKey="Ferrando, Sara" sort="Ferrando, Sara" uniqKey="Ferrando S" first="Sara" last="Ferrando">Sara Ferrando</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biology, University of Genoa, LIBiOM, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author><author><name sortKey="Gallus, Lorenzo" sort="Gallus, Lorenzo" uniqKey="Gallus L" first="Lorenzo" last="Gallus">Lorenzo Gallus</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biology, University of Genoa, LIBiOM, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author><author><name sortKey="Ravera, Silvia" sort="Ravera, Silvia" uniqKey="Ravera S" first="Silvia" last="Ravera">Silvia Ravera</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Biochemistry Laboratory, Department of Biology, University of Genoa, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author><author><name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>IFOM‐LAMBS/MicroScoBio, DIFI, University of Genoa, Genoa I‐16146</wicri:regionArea><wicri:noRegion>Genoa I‐16146</wicri:noRegion></affiliation></author><author><name sortKey="Ramoino, Paola" sort="Ramoino, Paola" uniqKey="Ramoino P" first="Paola" last="Ramoino">Paola Ramoino</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>DIPTERIS, University of Genoa, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author><author><name sortKey="Fasulo, Salvatore" sort="Fasulo, Salvatore" uniqKey="Fasulo S" first="Salvatore" last="Fasulo">Salvatore Fasulo</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Animal Biology and Marine Ecology, University of Messina, Messina 98166</wicri:regionArea><wicri:noRegion>Messina 98166</wicri:noRegion></affiliation></author><author><name sortKey="Tagliafierro, Grazia" sort="Tagliafierro, Grazia" uniqKey="Tagliafierro G" first="Grazia" last="Tagliafierro">Grazia Tagliafierro</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biology, University of Genoa, LIBiOM, Genoa I‐16132</wicri:regionArea><wicri:noRegion>Genoa I‐16132</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Microscopy Research and Technique</title><title level="j" type="abbrev">Microsc. Res. Tech.</title><idno type="ISSN">1059-910X</idno><idno type="eISSN">1097-0029</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-08">2010-08</date><biblScope unit="volume">73</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="797">797</biblScope><biblScope unit="page" to="802">802</biblScope></imprint><idno type="ISSN">1059-910X</idno></series><idno type="istex">2297E2743CDC00F707CFCA27458F2A01BBEA61BC</idno><idno type="DOI">10.1002/jemt.20822</idno><idno type="ArticleID">JEMT20822</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1059-910X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Dicentrarchus labrax</term><term>endomysial cells</term><term>immuno‐histochemistry</term><term>leptin</term><term>skeletal muscle</term><term>western blot</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The mammalian hormone, leptin, is mainly synthesized in adipose tissue along with other tissues. Leptin plays a role in numerous processes such as in the control of food intake, homeostasis, immune function and reproduction. In this study, we detected and localized leptin immunoreactivity to the muscle of early juvenile sea bass (Dicentrarchus labrax) by Western blot analysis and immunohistochemistry. A leptin immunopositive band with a molecular weight of ∼16 kDa, corresponding to mammalian leptin, was identified in trunk skeletal muscle homogenate. Furthermore, leptin immunopositive cells were detected in the endomysium of skeletal muscular fibers. These cells showed immunostained cytoplasmic granules and roundish and oval nuclei. The most intense immunostaining was observed in the endomysial space among the superficial red muscular fibers of the trunk. These findings suggest that in early juvenile sea bass, leptin is mostly produced by skeletal muscles. Therefore, during the developmental stage lacking adipose tissue, skeletal muscles can be considered an important source of leptin. As already suggested in mammals, we can hypothesize the potential roles of leptin not only in energy expenditure for muscle contraction but also during muscle differentiation and growth. Microsc. Res. Tech. 73:797–802, 2010. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Gambardella, Chiara" sort="Gambardella, Chiara" uniqKey="Gambardella C" first="Chiara" last="Gambardella">Chiara Gambardella</name></noRegion><name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name><name sortKey="Fasulo, Salvatore" sort="Fasulo, Salvatore" uniqKey="Fasulo S" first="Salvatore" last="Fasulo">Salvatore Fasulo</name><name sortKey="Ferrando, Sara" sort="Ferrando, Sara" uniqKey="Ferrando S" first="Sara" last="Ferrando">Sara Ferrando</name><name sortKey="Gallus, Lorenzo" sort="Gallus, Lorenzo" uniqKey="Gallus L" first="Lorenzo" last="Gallus">Lorenzo Gallus</name><name sortKey="Gambardella, Chiara" sort="Gambardella, Chiara" uniqKey="Gambardella C" first="Chiara" last="Gambardella">Chiara Gambardella</name><name sortKey="Ramoino, Paola" sort="Ramoino, Paola" uniqKey="Ramoino P" first="Paola" last="Ramoino">Paola Ramoino</name><name sortKey="Ravera, Silvia" sort="Ravera, Silvia" uniqKey="Ravera S" first="Silvia" last="Ravera">Silvia Ravera</name><name sortKey="Tagliafierro, Grazia" sort="Tagliafierro, Grazia" uniqKey="Tagliafierro G" first="Grazia" last="Tagliafierro">Grazia Tagliafierro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Eau/explor/LotaV3/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000447 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000447 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Eau
|area= LotaV3
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:2297E2743CDC00F707CFCA27458F2A01BBEA61BC
|texte= Leptin‐like immunoreactivity in the muscle of juvenile sea bass (Dicentrarchus labrax)
}}
| This area was generated with Dilib version V0.6.39. |  |